Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.314

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00		6.02E-02	5.99E-02
	B	25.00		5.03E-02	5.01E-02
	C	8.33		3.90E-02	4.20E-02
	D	2.78		2.42E-02	2.49E-02
	E	0.93		1.25E-02	1.22E-02
	F	0.31		5.10E-03	5.55E-03
	G	0.10	3.30E-03	2.70E-03	2.25E-03
	H	0.03	0	7.50E-04	7.50E-04

Yield (mg/mL): 1.314     Dilution Factor:  100×

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Protein purified on 3/24/2023 and assayed on 4/7/2023.
Data uploaded by aho007 and last updated on 2023-04-14 11:57:10.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		6.00E-04	7.50E-04
	B	48.3		7.50E-04	7.50E-04
	C	45.7	6.00E-04	7.50E-04
	D	42.4	3.30E-03	3.60E-03	3.15E-03
	E	37.7	1.88E-02	1.71E-02	1.62E-02
	F	33.6	1.97E-02	2.27E-02	1.77E-02
	G	31.3	2.09E-02	1.97E-02	2.19E-02
	H	30.0	2.28E-02	1.85E-02

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Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by aho007 and last updated on 2023-09-11 10:38:25.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.