Raw Data for BglB Variant Y396F

Expression Data

SDS-PAGE
 1.144

Kinetic Assay Data

slope of Y396F A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.83E-02 4.49E-02 4.73E-02
B 25.00 4.07E-02 4.19E-02 4.23E-02
C 8.33 3.29E-02 3.59E-02 3.74E-02
D 2.78 1.32E-02 1.49E-02 1.43E-02
E 0.93
F 0.31
G 0.10
H 0.03
 1.144      100×

Protein purified on 3/24/23 and assayed on 3/31/23.
Data uploaded by mamin016 and last updated on 2023-04-14 11:39:12.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 6.02E-02 5.99E-02
B 25.00 5.03E-02 5.01E-02
C 8.33 3.90E-02 4.20E-02
D 2.78 2.42E-02 2.49E-02
E 0.93 1.25E-02 1.22E-02
F 0.31 5.10E-03 5.55E-03
G 0.10 3.30E-03 2.70E-03 2.25E-03
H 0.03 0 7.50E-04 7.50E-04
 1.314      100×

Protein purified on 3/24/2023 and assayed on 4/7/2023.
Data uploaded by aho007 and last updated on 2023-04-14 11:57:10.


Thermostability Assay Data

slope of Y396F A420 nm (min−1)
T (°C) 1 2 3
A 50.0 9.00E-04 9.00E-04
B 48.3 9.00E-04 6.00E-04
C 45.7 1.05E-03 9.00E-04 7.50E-04
D 42.4 2.40E-03 2.55E-03
E 37.7 8.85E-03 8.25E-03 8.85E-03
F 33.6 1.31E-02 1.38E-02 1.43E-02
G 31.3 1.41E-02 1.55E-02 1.73E-02
H 30.0 1.50E-02 1.50E-02 1.65E-02

Protein purified on 3/24/23 and assayed on 3/31/23.
Data uploaded by mamin016 and last updated on 2023-04-18 09:46:20.


Reference Data for WT

slope of Y396F A420 nm (min−1)
T (°C) 1 2 3
A 50.0 6.00E-04 7.50E-04
B 48.3 7.50E-04 7.50E-04
C 45.7 6.00E-04 7.50E-04
D 42.4 3.30E-03 3.60E-03 3.15E-03
E 37.7 1.88E-02 1.71E-02 1.62E-02
F 33.6 1.97E-02 2.27E-02 1.77E-02
G 31.3 2.09E-02 1.97E-02 2.19E-02
H 30.0 2.28E-02 1.85E-02

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by aho007 and last updated on 2023-09-11 10:38:25.


Design Data

1.791

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.