Raw Data for BglB Variant L219Q

Expression Data

Kinetic Assay Data

Plate 20201217-2

slope of L219Q A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.242996	0.240505	0.236346
	B	18.75	0.188503	0.186919	0.186002
	C	4.69	0.10537	0.103152	0.101129
	D	1.17	0.0400382	0.0376897	0.0410576
	E	0.29	0.0114291	0.0108333	0.0118952
	F	0.07	0.00363212	0.00344667	0.00376788
	G	0.02		0.00227152	0.00222727
	H	0.00	1.25E-03	1.60E-03	1.31E-03

Yield / A_{280 nm}: 1.535     Dilution Factor:  100×

Download these data

Protein purified on 9/21/19 and assayed on 9/21/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

Reference Data for WT

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	8.86E-02	1.11E-01	8.31E-02
	B	18.75	7.30E-02	6.36E-02	5.65E-02
	C	4.69	5.06E-02	4.93E-02	4.65E-02
	D	1.17	2.51E-02	2.37E-02	2.22E-02
	E	0.29	9.62E-03	8.33E-03	8.61E-03
	F	0.07	2.82E-03	2.38E-03	2.40E-03
	G	0.02	7.90E-04	6.74E-04	7.26E-04
	H	0.00	1.88E-05	4.73E-05	0

Yield / A_{280 nm}: 1.03     Dilution Factor:  100×

Download these data

Protein purified on 9/21/19 and assayed on 9/21/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

Citations

The above data have been published in the following journal article(s):

• • Huang, P.; Chu, S.K.; Frizzo, H.N.; Connolly, M.P.; Caster, R.W.; & Siegel, J. B. (2020) “Evaluating Protein Engineering Thermostability Prediction Tools Using an Independently Generated Dataset..” ACS Omega 5(12), 6487–6493.