D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant K61P

Expression Data

Yield (mg/mL): 1.488

Kinetic Assay Data

**slope of K61P A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.36E-02	3.65E-02	3.74E-02
B	25.00	3.15E-02	3.23E-02	3.30E-02
C	8.33	2.45E-02	2.36E-02	2.40E-02
D	2.78	1.89E-02	1.91E-02	2.03E-02
E	0.93	7.95E-03	8.70E-03	9.30E-03
F	0.31	3.45E-03	4.05E-03	4.05E-03
G	0.10	1.65E-03	1.65E-03
H	0.03	9.00E-04	7.50E-04

Yield (mg/mL): 1.488 Dilution Factor: 100×

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by pgarr022 and last updated on 2024-05-23 09:40:35.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.27E-02	5.33E-02	5.34E-02
B	25.00	4.59E-02	4.59E-02	4.82E-02
C	8.33	3.86E-02	3.89E-02	3.86E-02
D	2.78	2.99E-02	2.72E-02	2.58E-02
E	0.93	1.17E-02	1.32E-02	1.20E-02
F	0.31	6.00E-03	6.00E-03	5.25E-03
G	0.10	2.70E-03	2.55E-03	2.55E-03
H	0.03	1.20E-03	0	7.50E-04

Yield (mg/mL): 1.683 Dilution Factor: 100×

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by pgarr022 and last updated on 2023-04-14 06:54:26.

Thermostability Assay Data

**slope of K61P A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0	6.00E-04	7.50E-04
B	48.0	4.50E-04		6.00E-04
C	45.7	6.00E-04	1.20E-03	7.50E-04
D	42.4	1.65E-03	1.65E-03	1.80E-03
E	37.7	1.04E-02	9.60E-03	9.45E-03
F	33.6	1.32E-02	1.58E-02	1.43E-02
G	31.3	1.32E-02	1.20E-02
H	30.0		1.41E-02	1.37E-02

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by pgarr022 and last updated on 2023-04-14 07:13:01.

Reference Data for WT

**slope of K61P A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	6.00E-04	6.00E-04
B	48.3	0	9.00E-04	0
C	45.7	9.00E-04	7.50E-04	7.50E-04
D	42.4	4.95E-03	5.10E-03	4.65E-03
E	37.7	1.86E-02	1.79E-02	1.67E-02
F	33.6	2.52E-02	2.42E-02	2.31E-02
G	31.3	2.63E-02	2.66E-02	2.48E-02
H	30.0	2.57E-02	2.36E-02	1.92E-02

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by pgarr022 and last updated on 2023-04-14 07:15:17.

Design Data

Rosetta Score for K61P: 11.704

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.