Raw Data for BglB Variant K61R
Expression Data
2.603
Kinetic Assay Data
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 7.92E-02 | 8.22E-02 | 9.00E-02 | ||
| B | 25.00 | 7.16E-02 | 7.62E-02 | 7.94E-02 | ||
| C | 8.33 | 5.57E-02 | 5.98E-02 | 6.17E-02 | ||
| D | 2.78 | 4.02E-02 | 4.49E-02 | 5.13E-02 | ||
| E | 0.93 | 1.92E-02 | 2.04E-02 | 2.13E-02 | ||
| F | 0.31 | 8.10E-03 | 8.70E-03 | 9.30E-03 | ||
| G | 0.10 | 3.15E-03 | 3.60E-03 | 3.60E-03 | ||
| H | 0.03 | 7.50E-04 | 7.50E-04 | 7.50E-04 |
Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by brianmena027 and last updated on 2023-04-14 06:58:41.
Reference Data for WT
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 5.27E-02 | 5.33E-02 | 5.34E-02 | ||
| B | 25.00 | 4.59E-02 | 4.59E-02 | 4.82E-02 | ||
| C | 8.33 | 3.86E-02 | 3.89E-02 | 3.86E-02 | ||
| D | 2.78 | 2.99E-02 | 2.72E-02 | 2.58E-02 | ||
| E | 0.93 | 1.17E-02 | 1.32E-02 | 1.20E-02 | ||
| F | 0.31 | 6.00E-03 | 6.00E-03 | 5.25E-03 | ||
| G | 0.10 | 2.70E-03 | 2.55E-03 | 2.55E-03 | ||
| H | 0.03 | 1.20E-03 | 0 | 7.50E-04 |
Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by pgarr022 and last updated on 2023-04-14 06:54:26.
Thermostability Assay Data
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 6.00E-04 | 9.00E-04 | 7.50E-04 | ||
| B | 48.3 | 6.00E-04 | 6.00E-04 | |||
| C | 45.7 | 1.20E-03 | 7.50E-04 | |||
| D | 42.4 | 2.13E-02 | 2.16E-02 | 2.13E-02 | ||
| E | 37.7 | 3.19E-01 | 3.27E-01 | 3.62E-01 | ||
| F | 33.6 | 4.11E-01 | 4.14E-01 | 4.19E-01 | ||
| G | 31.3 | 3.67E-01 | 3.32E-01 | 3.81E-01 | ||
| H | 30.0 | 4.34E-01 | 4.13E-01 | 4.31E-01 |
Reference Data for WT
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 6.00E-04 | 6.00E-04 | 6.00E-04 | ||
| B | 48.3 | 0 | 9.00E-04 | 0 | ||
| C | 45.7 | 9.00E-04 | 7.50E-04 | 7.50E-04 | ||
| D | 42.4 | 4.95E-03 | 5.10E-03 | 4.65E-03 | ||
| E | 37.7 | 1.86E-02 | 1.79E-02 | 1.67E-02 | ||
| F | 33.6 | 2.52E-02 | 2.42E-02 | 2.31E-02 | ||
| G | 31.3 | 2.63E-02 | 2.66E-02 | 2.48E-02 | ||
| H | 30.0 | 2.57E-02 | 2.36E-02 | 1.92E-02 |
Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by pgarr022 and last updated on 2023-04-14 07:15:17.
Design Data
Citations
The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.