Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.01

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	5.03E-02	5.31E-02	4.80E-02
	B	25.00	4.16E-02	4.57E-02	4.38E-02
	C	8.33	3.86E-02	4.19E-02	3.60E-02
	D	2.78	1.86E-02	1.93E-02	2.07E-02
	E	0.93	9.05E-03	9.80E-03	7.35E-03
	F	0.31	3.55E-03	3.60E-03	3.65E-03
	G	0.10	1.65E-03	1.85E-03	1.50E-03
	H	0.03	0	1.00E-04	1.50E-04

Yield (mg/mL): 1.01     Dilution Factor:  100×

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Protein purified on 3/30/2023 and assayed on 3/31/2023.
Data uploaded by VongphakdyK and last updated on 2023-04-13 10:36:47.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	7.00E-04		4.00E-04
	B	48.8	0	8.00E-04	4.00E-04
	C	46.5	1.30E-03	1.30E-03	1.60E-03
	D	42.5	1.16E-01	7.51E-02	9.32E-02
	E	37.6	2.90E-01	2.75E-01	3.32E-01
	F	33.8	2.51E-01	3.07E-01	3.30E-01
	G	31.7	3.19E-01	3.07E-01	3.16E-01
	H	30.0	3.30E-01		3.07E-01

Download these data

Protein purified on 3/30/2023 and assayed on 4/6/2023.
Data uploaded by VongphakdyK and last updated on 2023-04-13 10:55:45.

Citations

The unpublished data above were collected by students from Modesto Junior College. For inquiries, please contact Linda Brzezinski: <brzezinskil@yosemite.edu>.