Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	8.01E-02	7.91E-02	7.97E-02
	B	25.00	7.42E-02	7.12E-02	8.46E-02
	C	8.33	5.76E-02	5.41E-02	5.63E-02
	D	2.78	3.01E-02	3.81E-02	4.21E-02
	E	0.93	1.40E-02	1.48E-02	1.46E-02
	F	0.31	5.50E-03	5.55E-03	3.90E-03
	G	0.10	1.95E-03	2.05E-03	2.05E-03
	H	0.03	0	0	0

Yield (mg/mL): 1.59     Dilution Factor:  100×

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Protein purified on 3/30/2023 and assayed on 3/31/2023.
Data uploaded by dmg and last updated on 2023-04-13 10:26:05.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	4.00E-04	0	4.00E-04
	B	48.8	4.00E-04	0	4.00E-04
	C	46.5		1.10E-03	8.00E-04
	D	42.5	9.54E-02	1.22E-01	6.22E-02
	E	37.6	4.37E-01	4.30E-01	4.99E-01
	F	33.8		4.18E-01	4.03E-01
	G	31.2	2.41E-01	3.20E-01	3.05E-01
	H	30.0	3.28E-01	3.13E-01	3.72E-01

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Protein purified on 3/30/2023 and assayed on 4/6/2023.
Data uploaded by dmg and last updated on 2023-04-13 10:46:28.

Citations

The unpublished data above were collected by students from Modesto Junior College. For inquiries, please contact Linda Brzezinski: <brzezinskil@yosemite.edu>.