Raw Data for BglB Variant Q124K

Expression Data

Yield (mg/mL): 0.15

Kinetic Assay Data

slope of Q124K A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.05875	0.06880	0.06370
	B	25.00	0.04900	0.05015	0.05145
	C	8.33	0.04040	0.03930	0.03785
	D	2.78	0.02410	0.02100	0.01915
	E	0.93	0.008450	0.01010	0.007350
	F	0.31	0.004400	0.003650	0.002650
	G	0.10	0.001700	0.001500	0.001050
	H	0.03	0.0002500	0.0001500	0.0004000

Yield (mg/mL): 0.15     Dilution Factor:  10×

Download these data

Protein purified on 3/30/2023 and assayed on 3/31/2023.
Data uploaded by faustoe9 and last updated on 2023-04-13 10:27:34.

Thermostability Assay Data

slope of Q124K A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0	0.001000	0.001300
	B	48.8	0.0008000	0.0009000	0.0007000
	C	46.5	0.0007000	0.0007000	0.0007000
	D	42.5	0.001000	0.0008000	0.0009000
	E	37.6	0.001200	0.001800	0.002100
	F	33.8		0.002900	0.003100
	G	31.2	0.003200	0.003700	0.003900
	H	30.0		0.004400	0.004300

Download these data

Protein purified on 3/30/2023 and assayed on 4/6/2023.
Data uploaded by faustoe9 and last updated on 2023-04-13 11:01:58.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Citations

The unpublished data above were collected by students from Modesto Junior College. For inquiries, please contact Linda Brzezinski: <brzezinskil@yosemite.edu>.