Raw Data for BglB Variant Q124T

Expression Data

SDS-PAGE
 0.21

Kinetic Assay Data

slope of Q124T A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 7.73E-02 8.42E-02 8.08E-02
B 25.00 5.98E-02 6.62E-02 6.88E-02
C 8.33 4.11E-02 5.03E-02 5.59E-02
D 2.78 3.08E-02 2.81E-02 3.09E-02
E 0.93 1.39E-02 1.41E-02 1.40E-02
F 0.31 5.05E-03 5.20E-03 5.30E-03
G 0.10 2.00E-03 1.95E-03 2.20E-03
H 0.03 2.50E-04 0 0
 0.21      10×

Protein purified on 3/30/2023 and assayed on 3/31/2023.
Data uploaded by IsaiahK99 and last updated on 2023-04-13 10:23:56.



Thermostability Assay Data

slope of Q124T A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0006000 0 0.0008000
B 48.8 0.0006000 0 0.0005000
C 46.5 0.0006000 0 0.001100
D 42.5 0.001700 0.001800
E 37.6 0.01160 0.01090
F 33.8 0.01830 0.01980 0.01980
G 31.2 0.02670 0.02680 0.02630
H 30.0 0.02750 0.02710 0.02990

Protein purified on 3/30/2023 and assayed on 4/6/2023.
Data uploaded by IsaiahK99 and last updated on 2023-04-13 10:53:39.


Reference Data for WT

slope of Q124T A420 nm (min−1)
T (°C) 1 2 3
A 30.0 8.00E-04 3.60E-03
B