Raw Data for BglB Variant Q124P

Expression Data

SDS-PAGE
 1.21

Kinetic Assay Data

slope of Q124P A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.07100 0.07230 0.07250
B 25.00 0.07040 0.05770 0.06190
C 8.33 0.04980 0.04825 0.04920
D 2.78 0.02735 0.02485 0.02730
E 0.93 0.01155 0.01100 0.01065
F 0.31 0.004200 0.004300 0.004300
G 0.10 0.001650 0.001500
H 0.03 0 0.0002500 0
 1.21      100×

Protein purified on 3/30/2023 and assayed on 3/31/2023.
Data uploaded by Sergio and last updated on 2023-04-13 10:23:41.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 8.01E-02 7.91E-02 7.97E-02
B 25.00 7.42E-02 7.12E-02 8.46E-02
C 8.33 5.76E-02 5.41E-02 5.63E-02
D 2.78 3.01E-02 3.81E-02 4.21E-02
E 0.93 1.40E-02 1.48E-02 1.46E-02
F 0.31 5.50E-03 5.55E-03 3.90E-03
G 0.10 1.95E-03 2.05E-03 2.05E-03
H 0.03 0 0 0
 1.59      100×

Protein purified on 3/30/2023 and assayed on 3/31/2023.
Data uploaded by dmg and last updated on 2023-04-13 10:26:05.


Thermostability Assay Data

slope of Q124P A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0.0006000 0.0007000
B 48.8 0.0005000 0.0004000
C 46.5 0.0008000 0.0009000 0.001100
D 42.5 0.09520 0.09370
E 37.6 0.3785 0.2952 0.3872
F 33.8 0.4156 0.3620 0.2897
G 31.2 0.3799 0.4115
H 30.0 0.3617 0.4080 0.3861

Protein purified on 3/30/2023 and assayed on 4/6/2023.
Data uploaded by Sergio and last updated on 2023-04-13 10:50:36.


Reference Data for WT

slope of Q124P A420 nm (min−1)
T (°C) 1 2 3
A 50.0 4.00E-04 0 4.00E-04
B 48.8 4.00E-04 0 4.00E-04
C 46.5 1.10E-03 8.00E-04
D 42.5 9.54E-02 1.22E-01 6.22E-02
E 37.6 4.37E-01 4.30E-01 4.99E-01
F 33.8 4.18E-01 4.03E-01
G 31.2 2.41E-01 3.20E-01 3.05E-01
H 30.0 3.28E-01 3.13E-01 3.72E-01

Protein purified on 3/30/2023 and assayed on 4/6/2023.
Data uploaded by dmg and last updated on 2023-04-13 10:46:28.


Citations

The unpublished data above were collected by students from Modesto Junior College. For inquiries, please contact Linda Brzezinski: <brzezinskil@yosemite.edu>.