D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H194Y

Expression Data

Yield (mg/mL): 0.734

Kinetic Assay Data

**slope of H194Y A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.80E-01	3.93E-01	3.98E-01
B	25.00	3.03E-01	3.24E-01	3.42E-01
C	8.33	2.50E-01	2.59E-01	2.65E-01
D	2.78	1.59E-01	1.61E-01	1.55E-01
E	0.93	5.90E-02	6.11E-02	4.36E-02
F	0.31	2.36E-02	2.19E-02
G	0.10	6.75E-03	7.40E-03	5.25E-03
H	0.03	2.00E-04	2.50E-04	1.50E-04

Yield (mg/mL): 0.734 Dilution Factor: 10×

Protein purified on 3/30/2023 and assayed on 3/31/2023.
Data uploaded by priscilla2001 and last updated on 2023-04-13 10:23:52.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.03E-02	5.31E-02	4.80E-02
B	25.00	4.16E-02	4.57E-02	4.38E-02
C	8.33	3.86E-02	4.19E-02	3.60E-02
D	2.78	1.86E-02	1.93E-02	2.07E-02
E	0.93	9.05E-03	9.80E-03	7.35E-03
F	0.31	3.55E-03	3.60E-03	3.65E-03
G	0.10	1.65E-03	1.85E-03	1.50E-03
H	0.03	0	1.00E-04	1.50E-04

Yield (mg/mL): 1.01 Dilution Factor: 100×

Protein purified on 3/30/2023 and assayed on 3/31/2023.
Data uploaded by VongphakdyK and last updated on 2023-04-13 10:36:47.

Thermostability Assay Data

**slope of H194Y A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0	0	0.001800
B	48.8	0	0	0.0006000
C	46.5	0.0006000	0.0006000	0.0005000
D	42.5	0.009500		0.009000
E	37.6	0.1784	0.1791	0.1584
F	33.8	0.1908	0.1931	0.2400
G	31.7	0.1905	0.2032	0.1971
H	30.0	0.2113	0.2231	0.2353

Protein purified on 3/30/2023 and assayed on 4/6/2023.
Data uploaded by priscilla2001 and last updated on 2023-04-13 10:46:58.

Reference Data for WT

**slope of H194Y A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	7.00E-04		4.00E-04
B	48.8	0	8.00E-04	4.00E-04
C	46.5	1.30E-03	1.30E-03	1.60E-03
D	42.5	1.16E-01	7.51E-02	9.32E-02
E	37.6	2.90E-01	2.75E-01	3.32E-01
F	33.8	2.51E-01	3.07E-01	3.30E-01
G	31.7	3.19E-01	3.07E-01	3.16E-01
H	30.0	3.30E-01		3.07E-01

Protein purified on 3/30/2023 and assayed on 4/6/2023.
Data uploaded by VongphakdyK and last updated on 2023-04-13 10:55:45.

Citations

The unpublished data above were collected by students from Modesto Junior College. For inquiries, please contact Linda Brzezinski: <brzezinskil@yosemite.edu>.