D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H194Q

Expression Data

Yield (mg/mL): 0.16

Kinetic Assay Data

**slope of H194Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.94E-02	4.15E-02	3.56E-02
B	25.00	3.00E-02	2.97E-02	2.61E-02
C	8.33	2.28E-02	2.34E-02	2.08E-02
D	2.78	1.19E-02	1.28E-02	8.70E-03
E	0.93	7.20E-03	5.90E-03	4.65E-03
F	0.31	2.30E-03	2.25E-03	1.60E-03
G	0.10	1.00E-03	1.00E-03	7.50E-04
H	0.03	2.00E-04	1.50E-04	2.00E-04

Yield (mg/mL): 0.16 Dilution Factor: 10×

Protein purified on 3/30/2023 and assayed on 3/31/2023.
Data uploaded by Zalstad and last updated on 2023-04-13 10:21:06.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.03E-02	5.31E-02	4.80E-02
B	25.00	4.16E-02	4.57E-02	4.38E-02
C	8.33	3.86E-02	4.19E-02	3.60E-02
D	2.78	1.86E-02	1.93E-02	2.07E-02
E	0.93	9.05E-03	9.80E-03	7.35E-03
F	0.31	3.55E-03	3.60E-03	3.65E-03
G	0.10	1.65E-03	1.85E-03	1.50E-03
H	0.03	0	1.00E-04	1.50E-04

Yield (mg/mL): 1.01 Dilution Factor: 100×

Protein purified on 3/30/2023 and assayed on 3/31/2023.
Data uploaded by VongphakdyK and last updated on 2023-04-13 10:36:47.

Thermostability Assay Data

**slope of H194Q A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	8.00E-04	7.00E-04	4.00E-04
B	48.8	6.00E-04	5.00E-04
C	46.5	0	0	0
D	42.5		8.00E-04	8.00E-04
E	37.6	4.30E-03	4.40E-03
F	33.8		9.10E-03	9.00E-03
G	31.7	9.40E-03	9.50E-03	1.09E-02
H	30.0	1.00E-02	9.50E-03	8.10E-03

Protein purified on 3/30/2023 and assayed on 4/6/2023.
Data uploaded by Zalstad and last updated on 2023-04-13 10:46:50.

Reference Data for WT

**slope of H194Q A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	7.00E-04		4.00E-04
B	48.8	0	8.00E-04	4.00E-04
C	46.5	1.30E-03	1.30E-03	1.60E-03
D	42.5	1.16E-01	7.51E-02	9.32E-02
E	37.6	2.90E-01	2.75E-01	3.32E-01
F	33.8	2.51E-01	3.07E-01	3.30E-01
G	31.7	3.19E-01	3.07E-01	3.16E-01
H	30.0	3.30E-01		3.07E-01

Protein purified on 3/30/2023 and assayed on 4/6/2023.
Data uploaded by VongphakdyK and last updated on 2023-04-13 10:55:45.

Citations

The unpublished data above were collected by students from Modesto Junior College. For inquiries, please contact Linda Brzezinski: <brzezinskil@yosemite.edu>.