D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W325M

Expression Data

Yield (mg/mL): 1.174

Kinetic Assay Data

**slope of W325M A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	9.76E-02	1.01E-01	9.40E-02
B	25.00	6.87E-02	6.99E-02	6.66E-02
C	8.33	3.29E-02	3.31E-02	3.18E-02
D	2.78	9.70E-03	1.02E-02	9.80E-03
E	0.93	2.70E-03	2.55E-03	2.45E-03
F	0.31	7.00E-04	7.50E-04	6.50E-04
G	0.10	3.50E-04	4.00E-04	4.00E-04
H	0.03	3.50E-04	3.00E-04	3.50E-04

Yield (mg/mL): 1.174 Dilution Factor: 10×

Protein purified on 2/1/2023 and assayed on 2/3/2023.
Data uploaded by sdas407 and last updated on 2023-04-10 12:49:52.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.38E-01	1.74E-01	2.21E-01
B	25.00	1.67E-01	1.62E-01	1.61E-01
C	8.33	8.75E-02	8.53E-02	8.56E-02
D	2.78	2.65E-02	2.75E-02	2.76E-02
E	0.93	6.30E-03	6.30E-03	6.15E-03
F	0.31	1.50E-03	1.75E-03	1.45E-03
G	0.10	4.00E-04	6.50E-04	5.50E-04
H	0.03	0	0	3.50E-04

Yield (mg/mL): 2.079 Dilution Factor: 10×

Protein purified on 2/1/2023 and assayed on 2/3/2023.
Data uploaded by sdas407 and last updated on 2023-04-10 12:53:41.

Design Data

Rosetta Score for W325M: 3.453

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.