D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H328N

Yield (mg/mL): 0.0578

**slope of H328N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.70E-01	5.20E-01	4.80E-01
B	25.00	4.30E-01	4.30E-01	4.30E-01
C	8.33	3.50E-01	3.40E-01	3.50E-01
D	2.78	1.10E-01	1.10E-01	1.10E-01
E	0.93	3.00E-02	4.00E-02	4.00E-02
F	0.31	1.00E-02	1.00E-02	1.00E-02
G	0.10	7.69E-03	6.43E-03	6.43E-03
H	0.03	1.69E-03	2.30E-03	2.30E-03

Yield (mg/mL): 0.0578 Dilution Factor: 100×

Protein purified on 2/17/2023 and assayed on 3/31/2023.
Data uploaded by mrivaslucedu and last updated on 2023-03-31 11:19:44.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.35	0.31	0.34
B	25.00	0.22	0.19	0.18
C	8.33	0.1	0.1	0.1
D	2.78	0.06	0.06	0.06
E	0.93	0.03	0.03	0.03
F	0.31	0.01	0.01	0.01
G	0.10	0.005486	0.005614	0.00618
H	0.03	0.002563	0.002169	0.002263

Yield (mg/mL): 1.1449 Dilution Factor: 100×

Protein purified on 2/17/2023 and assayed on 3/3/2023.
Data uploaded by enielse and last updated on 2023-03-29 06:07:28.

**slope of H328N A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	30.0	1.10E-01		8.00E-02
B	31.3	1.00E-01	1.30E-01	9.00E-02
C	33.6
D	37.7		7.00E-02	7.00E-02
E	42.4	9.09E-03		1.00E-02
F	45.7	2.41E-03	1.85E-03	2.01E-03
G	48.3	1.38E-03	1.45E-03
H	50.0		2.22E-03	2.19E-03

Protein purified on 3/17/2023 and assayed on 3/31/2023.
Data uploaded by mrivaslucedu and last updated on 2023-03-31 11:48:38.

**slope of H328N A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		0.002614	0.002126
B	48.3	0.002391	0.00234	0.002126
C	45.7		0.003643	0.003369
D	42.4	0.03		0.03
E	37.7		0.1	0.11
F	33.6	0.1	0.1	0.11
G	31.3	0.14	0.14	0.15
H	30.0	0.15	0.12	0.13

Protein purified on 2/17/2023 and assayed on 3/17/2023.
Data uploaded by enielse and last updated on 2023-03-29 06:11:52.

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.