Raw Data for BglB Variant H223A

Expression Data

SDS-PAGE
 0.0769

Kinetic Assay Data

slope of H223A A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.00E-02 3.00E-02 4.00E-02
B 25.00 3.00E-02 3.00E-02 3.00E-02
C 8.33 2.00E-02 2.00E-02
D 2.78 1.00E-02 1.00E-02 1.00E-02
E 0.93 7.65E-03 5.84E-03 5.53E-03
F 0.31 3.42E-03 3.11E-03
G 0.10 2.43E-03 2.67E-03 2.66E-03
H 0.03 1.53E-03 1.31E-03
 0.0769      100×

Protein purified on 2/17/2023 and assayed on 3/3/2023.
Data uploaded by anhthy17 and last updated on 2023-03-31 10:45:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.35 0.36 0.31
B 25.00 0.19 0.17 0.17
C 8.33 0.1 0.09 0.09
D 2.78 0.05 0.05 0.05
E 0.93 0.02 0.02
F 0.31 0.01 0.01 0.009849
G 0.10 0.0054 0.005871 0.005006
H 0.03 0.003069 0.002743 0.00336
 1.1449      100×

Protein purified on 2/17/2023 and assayed on 3/3/2023.
Data uploaded by enielse and last updated on 2023-03-29 05:56:41.


Thermostability Assay Data

slope of H223A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 2.28E-03 2.49E-03 2.09E-03
B 48.3 2.91E-03 1.95E-03 2.64E-03
C 45.7 2.48E-03 2.47E-03
D 42.4 2.95E-03 2.91E-03 2.49E-03
E 37.7 1.29E-03 2.14E-03 2.89E-03
F 33.6 4.84E-03 5.13E-03 4.45E-03
G 31.3 6.41E-03 5.14E-03 4.32E-03
H 30.0 5.55E-03 6.03E-03 4.91E-03

Protein purified on 2/17/2023 and assayed on 3/17/2023.
Data uploaded by anhthy17 and last updated on 2023-03-31 10:55:20.


Reference Data for WT

slope of H223A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.002931 0.002889
B 48.3 0.001826 0.001894 0.001534
C 45.7 0.002623 0.0027 0.0027
D 42.4 0.02 0.02
E 37.7 0.07 0.08 0.07
F 33.6 0.1 0.11 0.12
G 31.3
H 30.0 0.11 0.14 0.11

Protein purified on 2/17/2023 and assayed on 3/17/2023.
Data uploaded by enielse and last updated on 2023-03-29 06:01:27.


Design Data

3.205

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.