Raw Data for BglB Variant T352V

Expression Data

SDS-PAGE
 0.123

Kinetic Assay Data

slope of T352V A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.95E-03 6.91E-03 5.50E-03
B 25.00 5.20E-03 5.89E-03 5.01E-03
C 8.33 4.75E-03 3.25E-03 5.90E-03
D 2.78 3.54E-03 3.62E-03
E 0.93 2.30E-03 3.21E-03 2.59E-03
F 0.31
G 0.10 2.49E-03 2.41E-03 2.43E-03
H 0.03
 0.123      100×

Protein purified on 2/17/23 and assayed on 3/3/23.
Data uploaded by rstanec and last updated on 2023-03-31 10:58:59.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.36 0.35 0.35
B 25.00 0.22 0.21 0.2
C 8.33 0.11 0.11
D 2.78 0.11 0.11
E 0.93 0.03 0.03 0.03
F 0.31 0.01 0.01 0.01
G 0.10 0.006189 0.006129 0.006197
H 0.03 0.00252 0.002477
 1.1449      100×

Protein purified on 2/17/2023 and assayed on 3/3/2023.
Data uploaded by enielse and last updated on 2023-03-29 06:21:00.



Thermostability Assay Data

slope of T352V A420 nm (min−1)
T (°C) 1 2 3
A 30.0 2.70E-03 2.88E-03
B