Raw Data for BglB Variant Y118F

Expression Data

SDS-PAGE
 0.6667

Kinetic Assay Data

slope of Y118F A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.60E-01 2.80E-01 3.20E-01
B 25.00 1.50E-01 1.50E-01 1.80E-01
C 8.33 1.00E-01 1.00E-01 1.10E-01
D 2.78 6.00E-02 6.00E-02 7.00E-02
E 0.93 3.00E-02 3.00E-02 3.00E-02
F 0.31 1.00E-02 1.00E-02 1.00E-02
G 0.10 6.15E-03 6.63E-03 7.14E-03
H 0.03 1.71E-03 1.57E-03 1.95E-03
 0.6667      100×

Protein purified on 2/17/2023 and assayed on 3/3/2023.
Data uploaded by kgordon8 and last updated on 2023-03-31 10:52:54.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.35 0.31 0.34
B 25.00 0.22 0.19 0.18
C 8.33 0.1 0.1 0.1
D 2.78 0.06 0.06 0.06
E 0.93 0.03 0.03 0.03
F 0.31 0.01 0.01 0.01
G 0.10 0.005486 0.005614 0.00618
H 0.03 0.002563 0.002169 0.002263
 1.1449      100×

Protein purified on 2/17/2023 and assayed on 3/3/2023.
Data uploaded by enielse and last updated on 2023-03-29 06:07:28.


Thermostability Assay Data

slope of Y118F A420 nm (min−1)
T (°C) 1 2 3
A 30.0 4.00E-02 5.00E-02 5.00E-02
B 31.3 6.00E-02 6.00E-02 7.00E-02
C 33.6 4.00E-02 4.00E-02 5.00E-02
D 37.7 3.00E-02 3.00E-02
E 42.4 4.81E-03 5.35E-03 4.96E-03
F 45.7 1.15E-03 1.38E-03
G 48.3 2.22E-03 1.93E-03 1.60E-03
H 50.0 1.97E-03 1.80E-03

Protein purified on 2/17/2023 and assayed on 3/17/2023.
Data uploaded by kgordon8 and last updated on 2023-03-31 11:06:26.


Reference Data for WT

slope of Y118F A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.002614 0.002126
B 48.3 0.002391 0.00234 0.002126
C 45.7 0.003643 0.003369
D 42.4 0.03 0.03
E 37.7 0.1 0.11
F 33.6 0.1 0.1 0.11
G 31.3 0.14 0.14 0.15
H 30.0 0.15 0.12 0.13

Protein purified on 2/17/2023 and assayed on 3/17/2023.
Data uploaded by enielse and last updated on 2023-03-29 06:11:52.


Design Data

1.884

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.