Raw Data for BglB Variant A80S

Expression Data

SDS-PAGE
 1.177

Kinetic Assay Data

slope of A80S A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.70E-01 4.30E-01 4.60E-01
B 25.00 3.60E-01 3.90E-01 3.60E-01
C 8.33 2.20E-01 2.40E-01 2.20E-01
D 2.78 8.00E-02 8.00E-02 9.00E-02
E 0.93 3.00E-02 3.00E-02 4.00E-02
F 0.31 1.00E-02 1.00E-02
G 0.10 6.83E-03 6.16E-03 6.71E-03
H 0.03 1.93E-03 1.71E-03
 1.177      100×

Protein purified on 2/17/2023 and assayed on 3/3/2023.
Data uploaded by mjames17 and last updated on 2023-03-31 10:35:10.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.35 0.31 0.34
B 25.00 0.22 0.19 0.18
C 8.33 0.1 0.1 0.1
D 2.78 0.06 0.06 0.06
E 0.93 0.03 0.03 0.03
F 0.31 0.01 0.01 0.01
G 0.10 0.005486 0.005614 0.00618
H 0.03 0.002563 0.002169 0.002263
 1.1449      100×

Protein purified on 2/17/2023 and assayed on 3/3/2023.
Data uploaded by enielse and last updated on 2023-03-29 06:07:28.


Thermostability Assay Data

slope of A80S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 1.72E-03 1.69E-03
B 48.3 1.11E-03 2.04E-03 2.67E-03
C 45.7 2.60E-03 2.73E-03
D 42.4 7.00E-02 6.00E-02 5.00E-02
E 37.7 2.20E-01 2.00E-01
F 33.6 2.20E-01 2.40E-01 2.20E-01
G 31.3 1.80E-01 2.00E-01 2.40E-01
H 30.0 2.60E-01 2.30E-01

Protein purified on 2/17/2023 and assayed on 3/17/2023.
Data uploaded by mjames17 and last updated on 2023-03-31 10:48:30.


Reference Data for WT

slope of A80S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.002614 0.002126
B 48.3 0.002391 0.00234 0.002126
C 45.7 0.003643 0.003369
D 42.4 0.03 0.03
E 37.7 0.1 0.11
F 33.6 0.1 0.1 0.11
G 31.3 0.14 0.14 0.15
H 30.0 0.15 0.12 0.13

Protein purified on 2/17/2023 and assayed on 3/17/2023.
Data uploaded by enielse and last updated on 2023-03-29 06:11:52.


Design Data

2.677

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.