Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.1449

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.35	0.31	0.34
	B	25.00	0.22	0.19	0.18
	C	8.33	0.1	0.1	0.1
	D	2.78	0.06	0.06	0.06
	E	0.93	0.03	0.03	0.03
	F	0.31	0.01	0.01	0.01
	G	0.10	0.005486	0.005614	0.00618
	H	0.03	0.002563	0.002169	0.002263

Yield (mg/mL): 1.1449     Dilution Factor:  100×

Download these data

Protein purified on 2/17/2023 and assayed on 3/3/2023.
Data uploaded by enielse and last updated on 2023-03-29 06:07:28.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		0.002614	0.002126
	B	48.3	0.002391	0.00234	0.002126
	C	45.7		0.003643	0.003369
	D	42.4	0.03		0.03
	E	37.7		0.1	0.11
	F	33.6	0.1	0.1	0.11
	G	31.3	0.14	0.14	0.15
	H	30.0	0.15	0.12	0.13

Download these data

Protein purified on 2/17/2023 and assayed on 3/17/2023.
Data uploaded by enielse and last updated on 2023-03-29 06:11:52.

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.