Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.1449

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.35	0.36	0.31
	B	25.00	0.19	0.17	0.17
	C	8.33	0.1	0.09	0.09
	D	2.78	0.05	0.05	0.05
	E	0.93		0.02	0.02
	F	0.31	0.01	0.01	0.009849
	G	0.10	0.0054	0.005871	0.005006
	H	0.03	0.003069	0.002743	0.00336

Yield (mg/mL): 1.1449     Dilution Factor:  100×

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Protein purified on 2/17/2023 and assayed on 3/3/2023.
Data uploaded by enielse and last updated on 2023-03-29 05:56:41.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		0.002931	0.002889
	B	48.3	0.001826	0.001894	0.001534
	C	45.7	0.002623	0.0027	0.0027
	D	42.4	0.02	0.02
	E	37.7	0.07	0.08	0.07
	F	33.6	0.1	0.11	0.12
	G	31.3
	H	30.0	0.11	0.14	0.11

Download these data

Protein purified on 2/17/2023 and assayed on 3/17/2023.
Data uploaded by enielse and last updated on 2023-03-29 06:01:27.

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.