Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.1449

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.48	0.45	0.44
	B	25.00	0.4	0.44	0.39
	C	8.33	0.34	0.31	0.28
	D	2.78	0.11	0.1	0.1
	E	0.93	0.04	0.04	0.04
	F	0.31	0.02	0.02	0.02
	G	0.10	0.006077	0.006857	0.008169
	H	0.03	0.003531	0.001286	0.001903

Yield (mg/mL): 1.1449     Dilution Factor:  100×

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Protein purified on 2/17/2023 and assayed on 3/3/2023.
Data uploaded by enielse and last updated on 2023-03-28 10:55:15.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.002134	0.002443	0.001466
	B	48.3	0.001817		0.002469
	C	45.7	0.003857	0.003677
	D	42.4	0.02	0.02
	E	37.7	0.07	0.1
	F	33.6	0.12	0.12	0.1
	G	31.3	0.09	0.12	0.14
	H	30.0		0.13	0.14

Download these data

Protein purified on 2/17/2023 and assayed on 3/17/2023.
Data uploaded by enielse and last updated on 2023-03-28 11:02:34.

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.