Raw Data for BglB Variant H178S

Expression Data

Kinetic Assay Data

Plate 20201217-1

slope of H178S A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.00562788	0.00525818	0.00551333
	B	18.75	0.00360182	0.00352909	0.00354121
	C	4.69	0.00160545	0.00160727	0.00167091
	D	1.17	0.000565455	0.000495152
	E	0.29	0.000172727	0.000167273
	F	0.07	6.55E-05	6.91E-05	6.06E-05
	G	0.02	3.82E-05	3.76E-05
	H	0.00	2.91E-05	3.39E-05	2.48E-05

Yield / A_{280 nm}: 0.095     Dilution Factor:  10×

Download these data

Protein purified on 9/21/19 and assayed on 9/21/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

Reference Data for WT

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.83E-02	3.83E-02	3.67E-02
	B	18.75	2.87E-02	2.90E-02	2.75E-02
	C	4.69	1.81E-02	1.85E-02	1.75E-02
	D	1.17	8.66E-03	7.97E-03	6.91E-03
	E	0.29	2.80E-03	2.45E-03	2.24E-03
	F	0.07	8.14E-04	6.82E-04	6.20E-04
	G	0.02	2.75E-04	2.06E-04	2.08E-04
	H	0.00	8.12E-05	6.67E-05	7.21E-05

Yield / A_{280 nm}: 0.207     Dilution Factor:  50×

Download these data

Protein purified on 9/21/19 and assayed on 9/21/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

Citations

The above data have been published in the following journal article(s):

• • Huang, P.; Chu, S.K.; Frizzo, H.N.; Connolly, M.P.; Caster, R.W.; & Siegel, J. B. (2020) “Evaluating Protein Engineering Thermostability Prediction Tools Using an Independently Generated Dataset..” ACS Omega 5(12), 6487–6493.