D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N404L

Expression Data

Yield (mg/mL): 2.778

Kinetic Assay Data

**slope of N404L A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.01225	0.01195	0.01410
B	25.00	0.008200	0.007850	0.008350
C	8.33	0.004200	0.003950	0.003900
D	2.78	0.001850	0.001450	0.001850
E	0.93	0.0007500	0.0007000	0.0006500
F	0.31	0.0005500	0.0005000	0.0004500
G	0.10		0.0002500	0.0003000
H	0.03		0.0000000	0.0000000

Yield (mg/mL): 2.778 Dilution Factor: 100×

Protein purified on 2/28/23 and assayed on 3/1/23.
Data uploaded by anarsam and last updated on 2023-03-24 01:49:09.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2389	0.2363	0.2376
B	25.00	0.2200	0.2289	0.2490
C	8.33	0.1705	0.1842	0.1862
D	2.78	0.09585	0.1003	0.1156
E	0.93	0.02575	0.03910	0.04405
F	0.31	0.01675	0.01690	0.01680
G	0.10	0.005650	0.005600	0.005450
H	0.03	0.0003000	0.0003000	0

Yield (mg/mL): 2.532 Dilution Factor: 100×

Protein purified on 2/28/23 and assayed on 3/1/23.
Data uploaded by anarsam and last updated on 2023-03-23 14:13:16.

Design Data

Rosetta Score for N404L: -1.689

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.