D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E406Q

Expression Data

Yield (mg/mL): 1.502

Kinetic Assay Data

**slope of E406Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.12E-02	1.99E-02	1.98E-02
B	25.00	1.08E-02	9.40E-03	1.12E-02
C	8.33	4.20E-03	3.85E-03	3.90E-03
D	2.78	1.60E-03	1.55E-03	1.70E-03
E	0.93	6.00E-04	7.50E-04	6.50E-04
F	0.31	3.00E-04	3.00E-04
G	0.10	3.00E-04	3.00E-04	3.50E-04
H	0.03	2.00E-04	0	1.50E-04

Yield (mg/mL): 1.502 Dilution Factor: 10×

Protein purified on 11/3/2022 and assayed on 11/4/2022.
Data uploaded by anng19 and last updated on 2023-02-01 15:11:01.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		2.35E-01	2.15E-01
B	25.00		1.37E-01	1.52E-01
C	8.33	6.51E-02	1.55E-01	1.32E-01
D	2.78	4.51E-02	7.62E-02	5.99E-02
E	0.93		2.02E-02	2.17E-02
F	0.31	1.10E-02	3.25E-03	7.40E-03
G	0.10		3.35E-03	2.70E-03
H	0.03		4.50E-04	4.50E-04

Yield (mg/mL): 1.842 Dilution Factor: 100×

Protein purified on 11/3/2022 and assayed on 11/4/2022.
Data uploaded by anng19 and last updated on 2023-01-30 16:58:09.

Design Data

Rosetta Score for E406Q: 4.305

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.