Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.091

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	4.10E-03	4.50E-03	4.00E-03
	B	25.00	3.50E-03	3.90E-03	3.40E-03
	C	8.33	2.90E-03	3.10E-03	3.40E-03
	D	2.78	1.60E-03	1.60E-03	1.90E-03
	E	0.93	7.00E-04	7.00E-04	8.00E-04
	F	0.31	3.00E-04	3.00E-04	3.00E-04
	G	0.10	1.00E-04	7.00E-05	1.00E-04
	H	0.03		4.00E-05	3.00E-05

Yield (mg/mL): 0.091     Dilution Factor:  100×

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Protein purified on 11/22/2022 and assayed on 12/2/2022.
Data uploaded by ZacB2022 and last updated on 2022-12-06 07:00:42.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	30.0	1.86E-01	2.00E-01	1.88E-01
	B	31.3	1.86E-01	1.84E-01	1.91E-01
	C	33.6	1.63E-01	1.79E-01	1.29E-01
	D	37.7	1.58E-01	1.49E-01	1.52E-01
	E	42.4	3.75E-02	3.94E-02	4.55E-02
	F	45.7	4.00E-04	4.00E-04	5.00E-04
	G	48.3	6.00E-05	9.00E-05	0.00E+00
	H	50.0	7.00E-06		0.00E+00

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Protein purified on 11/22/2022 and assayed on 12/2/2022.
Data uploaded by Evan and last updated on 2022-12-06 07:58:12.

Citations

The unpublished data above were collected by students from Campbell University. For inquiries, please contact Evan Reynolds: <ereynolds@campbell.edu>.