D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H194L

Expression Data

Yield (mg/mL): 0.19

Kinetic Assay Data

**slope of H194L A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	7.65E-03	6.59E-03	6.43E-03
B	25.00
C	8.33	8.42E-03		7.21E-03
D	2.78	4.99E-03	5.07E-03	4.26E-03
E	0.93	2.41E-03	2.43E-03	2.53E-03
F	0.31	1.01E-03	1.05E-03	9.87E-04
G	0.10	3.48E-04	3.46E-04	3.81E-04
H	0.03		2.91E-05

Yield (mg/mL): 0.19 Dilution Factor: 100×

Protein purified on 11/9/22 and assayed on 11/9/22.
Data uploaded by DylanMR and last updated on 2022-11-21 18:25:53.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.11E-02	6.53E-02	6.93E-02
B	25.00	5.83E-02	6.20E-02	7.00E-02
C	8.33	4.64E-02	4.80E-02	4.93E-02
D	2.78	2.75E-02	2.94E-02	2.73E-02
E	0.93	1.30E-02	1.25E-02	1.18E-02
F	0.31	5.11E-03	5.63E-03	4.36E-03
G	0.10	1.86E-03	1.96E-03	1.77E-03
H	0.03	2.73E-05	1.45E-05	2.30E-05

Yield (mg/mL): 0.98 Dilution Factor: 100×

Protein purified on 11/9/2022 and assayed on 11/9/2022.
Data uploaded by ShreyaT and last updated on 2022-11-16 11:20:51.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of H194L A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	5.00E-04	5.00E-04	6.00E-04
B	48.3	3.00E-04	4.00E-04	5.00E-04
C	45.7	7.00E-04	5.00E-04	6.00E-04
D	42.4	4.60E-03	4.60E-03	4.10E-03
E	37.7	2.13E-02		2.11E-02
F	33.6	1.94E-02	1.44E-02	1.64E-02
G	31.3	1.89E-02	2.03E-02	1.97E-02
H	30.0	1.56E-02	1.80E-02	2.00E-02

Protein purified on 11/9/2022 and assayed on 11/9/2022.
Data uploaded by ShreyaT and last updated on 2022-11-16 11:36:44.

Design Data

Rosetta Score for H194L: 28.931

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.