Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.29E-01	1.22E-01	1.60E-01
	B	25.00	1.31E-01	9.52E-02	1.83E-01
	C	8.33	1.07E-01	7.84E-02	9.78E-02
	D	2.78	7.70E-02	5.26E-02	4.06E-02
	E	0.93	2.35E-02	3.12E-02	2.49E-02
	F	0.31	9.70E-03	9.75E-03	1.06E-02
	G	0.10	3.95E-03	4.95E-03	3.40E-03
	H	0.03	3.50E-04	0	0

Yield (mg/mL): 1.306     Dilution Factor:  100×

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Protein purified on 11/9/2022 and assayed on 11/10/2022.
Data uploaded by BryantLuu and last updated on 2022-11-19 15:18:00.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	4.00E-04	3.00E-04
	B	48.3	4.00E-04	0	4.00E-04
	C	45.7	1.15E-03	6.50E-04	8.00E-04
	D	42.4	1.46E-02	1.36E-02	1.43E-02
	E	37.7	5.10E-02	4.49E-02	4.02E-02
	F	33.6	5.34E-02	5.29E-02	5.42E-02
	G	31.3	6.68E-02	5.32E-02	6.24E-02
	H	30.0	6.62E-02	5.58E-02	5.45E-02

Download these data

Protein purified on 11/9/2022 and assayed on 11/10/2022.
Data uploaded by BryantLuu and last updated on 2022-11-20 14:40:06.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Peishan: <peihuang@ucdavis.edu>.