Raw Data for BglB Variant Q124D

Expression Data

SDS-PAGE
 0.75

Kinetic Assay Data

slope of Q124D A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.65E-02 3.93E-02 3.92E-02
B 25.00 3.98E-02 3.75E-02 3.87E-02
C 8.33 2.71E-02 2.97E-02 3.12E-02
D 2.78 1.33E-02 1.51E-02
E 0.93 5.70E-03 5.65E-03 6.20E-03
F 0.31 2.50E-03 2.25E-03 2.45E-03
G 0.10 9.50E-04 1.00E-03 1.05E-03
H 0.03 3.00E-04 6.50E-04 0
 0.75      100×

Protein purified on 11/2/2022 and assayed on 11/9/2022.
Data uploaded by vincentp and last updated on 2022-11-18 22:43:56.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.05005 0.05545 0.0509
B 25.00 0.0451 0.05685 0.0494
C 8.33 0.04285 0.0428 0.041
D 2.78 0.01635 0.01995 0.0179
E 0.93 0.0095 0.008151 0.00845
F 0.31 0.00395 0.0033 0.00235
G 0.10 0.0014 0.00125
H 0.03 0 0.0002 0.00035
 0.56      100×

Protein purified on 11/2/2022 and assayed on 11/9/2022.
Data uploaded by jannahal and last updated on 2022-11-18 19:11:00.


Thermostability Assay Data

slope of Q124D A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 0.0006000
B 48.3 0.0005000 0 0
C 45.7 0.0006000 0 0
D 42.4 0.0008000 0.0008000
E 37.7 0.003200 0.002700
F 33.6 0.006300 0.005100 0.005300
G 31.3 0.009000 0.007300 0.006500
H 30.0 0.007900 0.007300 0.007400

Protein purified on 11/2/2022 and assayed on 11/9/2022.
Data uploaded by vincentp and last updated on 2022-11-18 22:53:11.


Reference Data for WT

slope of Q124D A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 0
B 48.3 0 0 0
C 45.7 0 0.0007 0.0006
D 42.4 0.0019 0.0021
E 37.7 0.0124 0.0135 0.0109
F 33.6 0.012 0.0149 0.0157
G 31.3 0.0101 0.013 0.0164
H 30.0 0.0084 0.0085

Protein purified on 11/2/2022 and assayed on 11/9/2022.
Data uploaded by jannahal and last updated on 2022-11-18 19:16:13.


Design Data

10.075

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.