Raw Data for BglB Variant S302W

Expression Data

SDS-PAGE
 4.935

Kinetic Assay Data

slope of S302W A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.84E-01 1.88E-01 1.90E-01
B 25.00 1.63E-01 1.55E-01 1.67E-01
C 8.33 1.13E-01 9.87E-02 1.12E-01
D 2.78 6.78E-02 5.46E-02 7.83E-02
E 0.93 3.03E-02 2.19E-02 3.02E-02
F 0.31 1.17E-02 1.19E-02
G 0.10 4.50E-03 3.15E-03 4.50E-03
H 0.03 7.50E-04 6.00E-04 6.00E-04
 4.935      100×

Protein purified on 10/21/2022 and assayed on 10/28/2022.
Data uploaded by gcorr003 and last updated on 2022-12-01 15:59:14.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3587 0.3504 0.3458
B 25.00 0.2868 0.3096 0.3092
C 8.33 0.2193 0.2441 0.2356
D 2.78 0.1266 0.1298 0.1290
E 0.93 0.05670 0.05880 0.05925
F 0.31 0.02340 0.02235 0.02235
G 0.10 0.008250 0.008400 0.008250
H 0.03 0.001050 0.001050
 6.878      100×

Protein purified on 10/21/2022 and assayed on 10/28/2022.
Data uploaded by nnaja003 and last updated on 2022-12-01 15:45:42.


Thermostability Assay Data

slope of S302W A420 nm (min−1)
T (°C) 1 2 3
A 50.0 7.50E-04 7.50E-04
B 48.3 7.50E-04 7.50E-04
C 45.7 6.00E-04 6.00E-04
D 42.4 2.70E-03 2.55E-03 2.70E-03
E 37.7 7.67E-02 5.69E-02 7.11E-02
F 33.6 8.63E-02 9.33E-02 8.46E-02
G 31.3 9.23E-02 9.08E-02 8.19E-02
H 30.0 8.52E-02 7.79E-02

Protein purified on 10/21/2022 and assayed on 10/28/2022.
Data uploaded by gcorr003 and last updated on 2024-08-01 14:41:04.


Reference Data for WT

slope of S302W A420 nm (min−1)
T (°C) 1 2 3
A 50.0
B 48.3
C 45.7
D 42.4 0.01305 0.01200 0.01245
E 37.7 0.1628 0.1671 0.1490
F 33.6 0.1326 0.1335 0.1318
G 31.2 0.1919 0.1962 0.1778
H 30.0 0.1761 0.1761

Protein purified on and assayed on .
Data uploaded by nnaja003 and last updated on 2023-09-11 10:33:32.


Design Data

6.224

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.