Raw Data for BglB Variant T352V

Expression Data

Yield (mg/mL): 0.045

Kinetic Assay Data

slope of T352V A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.00E-02	9.36E-03	1.00E-02
	B	25.00	1.00E-02	1.00E-02	1.00E-02
	C	8.33	7.35E-03	9.16E-03	8.11E-03
	D	2.78	5.86E-03	4.57E-03	4.30E-03
	E	0.93	2.47E-03	2.99E-03	2.13E-03
	F	0.31	1.53E-03	1.71E-03
	G	0.10	1.71E-03	1.75E-03	1.96E-03
	H	0.03	5.80E-04	1.02E-03	1.18E-03

Yield (mg/mL): 0.045     Dilution Factor:  10×

Download these data

Protein purified on 10/7/2022 and assayed on 10/21/2022.
Data uploaded by kashar and last updated on 2022-11-18 12:45:06.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

slope of T352V A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.002597	0.002306	0.002177
	B	48.3	0.002854	0.003137
	C	45.7	0.003377	0.00318
	D	42.4	0.003411	0.00372	0.003891
	E	37.7	0.02	0.02	0.02
	F	33.6		0.03	0.03
	G	31.3	0.03	0.03	0.03
	H	30.0	0.03	0.03

Download these data

Protein purified on 10/7/2022 and assayed on 10/28/2022.
Data uploaded by enielse and last updated on 2022-11-18 11:39:14.

Design Data

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.