Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 6.878

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.3587	0.3504	0.3458
	B	25.00	0.2868	0.3096	0.3092
	C	8.33	0.2193	0.2441	0.2356
	D	2.78	0.1266	0.1298	0.1290
	E	0.93	0.05670	0.05880	0.05925
	F	0.31	0.02340	0.02235	0.02235
	G	0.10	0.008250	0.008400	0.008250
	H	0.03		0.001050	0.001050

Yield (mg/mL): 6.878     Dilution Factor:  100×

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Protein purified on 10/21/2022 and assayed on 10/28/2022.
Data uploaded by nnaja003 and last updated on 2022-12-01 15:45:42.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0
	B	48.3
	C	45.7
	D	42.4	0.01305	0.01200	0.01245
	E	37.7	0.1628	0.1671	0.1490
	F	33.6	0.1326	0.1335	0.1318
	G	31.2	0.1919	0.1962	0.1778
	H	30.0	0.1761	0.1761

Download these data

Protein purified on and assayed on .
Data uploaded by nnaja003 and last updated on 2023-09-11 10:33:32.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.