Raw Data for BglB Variant S302W

Expression Data

SDS-PAGE
 6.307

Kinetic Assay Data

slope of S302W A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.04E-01 4.47E-01 3.39E-01
B 25.00 4.38E-01 5.25E-01
C 8.33 3.68E-01 2.33E-01 3.08E-01
D 2.78 1.45E-01 1.33E-01 1.33E-01
E 0.93 1.42E-01 1.00E-01
F 0.31 3.05E-02 3.29E-02
G 0.10 1.74E-02 1.25E-02 1.31E-02
H 0.03 0 0
 6.307      100×

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by sofiacg512 and last updated on 2024-08-01 14:43:38.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3587 0.3504 0.3458
B 25.00 0.2868 0.3096 0.3092
C 8.33 0.2193 0.2441 0.2356
D 2.78 0.1266 0.1298 0.1290
E 0.93 0.05670 0.05880 0.05925
F 0.31 0.02340 0.02235 0.02235
G 0.10 0.008250 0.008400 0.008250
H 0.03 0.001050 0.001050
 6.878      100×

Protein purified on 10/21/2022 and assayed on 10/28/2022.
Data uploaded by nnaja003 and last updated on 2022-12-01 15:45:42.


Thermostability Assay Data

slope of S302W A420 nm (min−1)
T (°C) 1 2 3
A 50.0 6.00E-04 7.50E-04 6.00E-04
B 48.3 4.50E-04 7.50E-04
C 45.7 7.50E-04 7.50E-04 7.50E-04
D 42.4 5.85E-03 5.40E-03 5.10E-03
E 37.7 1.39E-01 1.36E-01 1.31E-01
F 33.6 1.57E-01 1.76E-01 1.70E-01
G 31.3 1.70E-01 1.66E-01 1.71E-01
H 30.0 1.78E-01 2.02E-01 1.64E-01

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by sofiacg512 and last updated on 2022-11-18 12:51:36.


Reference Data for WT

slope of S302W A420 nm (min−1)
T (°C) 1 2 3
A 50.0
B 48.3
C 45.7
D 42.4 0.01305 0.01200 0.01245
E 37.7 0.1628 0.1671 0.1490
F 33.6 0.1326 0.1335 0.1318
G 31.2 0.1919 0.1962 0.1778
H 30.0 0.1761 0.1761

Protein purified on and assayed on .
Data uploaded by nnaja003 and last updated on 2023-09-11 10:33:32.


Design Data

6.224

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.