D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant S302D

Expression Data

Yield (mg/mL): 6.771

Kinetic Assay Data

**slope of S302D A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.39E-01	1.30E-01	1.12E-01
B	25.00	8.85E-02	9.08E-02	8.94E-02
C	8.33	5.70E-02	5.48E-02	5.49E-02
D	2.78	2.40E-02	2.63E-02	2.40E-02
E	0.93	1.04E-02	1.17E-02	1.08E-02
F	0.31	3.90E-03	3.90E-03	4.20E-03
G	0.10	1.65E-03	1.80E-03
H	0.03	0	0	0

Yield (mg/mL): 6.771 Dilution Factor: 100×

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by tylerroberts and last updated on 2022-11-18 12:37:17.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3587	0.3504	0.3458
B	25.00	0.2868	0.3096	0.3092
C	8.33	0.2193	0.2441	0.2356
D	2.78	0.1266	0.1298	0.1290
E	0.93	0.05670	0.05880	0.05925
F	0.31	0.02340	0.02235	0.02235
G	0.10	0.008250	0.008400	0.008250
H	0.03		0.001050	0.001050

Yield (mg/mL): 6.878 Dilution Factor: 100×

Protein purified on 10/21/2022 and assayed on 10/28/2022.
Data uploaded by nnaja003 and last updated on 2022-12-01 15:45:42.

Thermostability Assay Data

**slope of S302D A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0	4.50E-04	0
B	48.3	0	0	6.00E-04
C	45.7	6.00E-04	6.00E-04	6.00E-04
D	42.4	3.60E-03	3.00E-03	2.85E-03
E	37.7	2.90E-02	2.78E-02	2.73E-02
F	33.6	3.36E-02	3.41E-02	3.30E-02
G	31.3	3.56E-02	3.60E-02	3.35E-02
H	30.0	3.66E-02	3.93E-02	3.36E-02

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by tylerroberts and last updated on 2022-11-18 12:45:06.

Reference Data for WT

**slope of S302D A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	7.50E-04	6.00E-04
B	48.0	6.00E-04	0	4.50E-04
C	46.0	6.00E-04	1.05E-03	7.50E-04
D	42.0	6.60E-03	6.45E-03	5.85E-03
E	38.0	3.74E-02	3.84E-02	3.56E-02
F	34.0	4.25E-02	4.16E-02	4.13E-02
G	31.0	4.98E-02	5.04E-02	4.80E-02
H	30.0	4.10E-02	4.35E-02	3.98E-02

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by Seaotter01 and last updated on 2022-11-18 08:12:14.

Design Data

Rosetta Score for S302D: 5.901

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.