Raw Data for BglB Variant S302F

Expression Data

SDS-PAGE
 6.64

Kinetic Assay Data

slope of S302F A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.2689 0.2775 0.2712
B 25.00 0.2220 0.2184 0.2258
C 8.33 0.1660 0.1764 0.1821
D 2.78 0.09630 0.1065 0.1058
E 0.93 0.04635 0.04935 0.04530
F 0.31 0.01935 0.02175 0.01890
G 0.10 0.007500 0.008402 0.006900
H 0.03 0 0.0006000 0
 6.64      100×

Protein purified on 10/21/2022 and assayed on 10/21/2022.
Data uploaded by Ememgini and last updated on 2022-11-18 12:36:53.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3587 0.3504 0.3458
B 25.00 0.2868 0.3096 0.3092
C 8.33 0.2193 0.2441 0.2356
D 2.78 0.1266 0.1298 0.1290
E 0.93 0.05670 0.05880 0.05925
F 0.31 0.02340 0.02235 0.02235
G 0.10 0.008250 0.008400 0.008250
H 0.03 0.001050 0.001050
 6.878      100×

Protein purified on 10/21/2022 and assayed on 10/28/2022.
Data uploaded by nnaja003 and last updated on 2022-12-01 15:45:42.


Thermostability Assay Data

slope of S302F A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 0.0006000
B 48.3 0.0004500 0.0006001 0
C 45.7 0.0006000 0.0006000 0.0007500
D 42.4 0.005850 0.005850 0.006000
E 37.7 0.1133 0.1112 0.1139
F 33.6 0.1268 0.1296 0.1278
G 31.3 0.1295 0.1323 0.1282
H 30.0 0.1223 0.1212 0.1221

Protein purified on 10/21/2022 and assayed on 10/21/2022.
Data uploaded by Ememgini and last updated on 2022-11-18 12:44:12.


Reference Data for WT

slope of S302F A420 nm (min−1)
T (°C) 1 2 3
A 50.0 6.00E-04 7.50E-04 6.00E-04
B 48.0 6.00E-04 0 4.50E-04
C 46.0 6.00E-04 1.05E-03 7.50E-04
D 42.0 6.60E-03 6.45E-03 5.85E-03
E 38.0 3.74E-02 3.84E-02 3.56E-02
F 34.0 4.25E-02 4.16E-02 4.13E-02
G 31.0 4.98E-02 5.04E-02 4.80E-02
H 30.0 4.10E-02 4.35E-02 3.98E-02

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by Seaotter01 and last updated on 2022-11-18 08:12:14.


Design Data

6.165

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.