Raw Data for BglB Variant A80S

Expression Data

SDS-PAGE
 0.6612

Kinetic Assay Data

slope of A80S A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.50E-01 3.50E-01 3.50E-01
B 25.00 3.00E-01 2.80E-01 2.80E-01
C 8.33 1.60E-01 1.50E-01 2.00E-01
D 2.78 6.00E-02 5.00E-02 5.00E-02
E 0.93 2.00E-02 2.00E-02
F 0.31 9.63E-03 9.93E-03 8.12E-03
G 0.10 3.91E-03 4.18E-03 4.29E-03
H 0.03 9.27E-04 1.14E-03 1.10E-03
 0.6612      100×

Protein purified on 10/7/22 and assayed on 10/21/22.
Data uploaded by abesa and last updated on 2022-11-18 12:41:16.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.09 0.1 0.09
B 25.00 0.06 0.06 0.07
C 8.33 0.05 0.05 0.04
D 2.78 0.03 0.03 0.03
E 0.93 0.01 0.01 0.01
F 0.31 0.005393 0.006307 0.004977
G 0.10 0.002707 0.002741 0.003457
H 0.03 0.000988636 0.001036
 0.309      100×

Protein purified on 10/7/2022 and assayed on 10/21/2022.
Data uploaded by enielse and last updated on 2022-11-22 16:27:41.


Thermostability Assay Data

slope of A80S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 3.10E-03 2.95E-03
B 48.3 3.33E-03 2.49E-03 5.19E-03
C 45.7 1.83E-03 3.06E-03 5.19E-03
D 42.4 9.87E-03 2.00E-02 3.00E-02
E 37.7 2.00E-02 4.00E-02 5.00E-02
F 33.6 4.00E-02 4.00E-02 5.00E-02
G 31.3 5.00E-02 5.00E-02 6.00E-02
H 30.0 5.00E-02 6.00E-02 6.00E-02

Protein purified on 10/7/22 and assayed on 10/28/22.
Data uploaded by abesa and last updated on 2022-11-18 12:50:30.


Reference Data for WT

slope of A80S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.002597 0.002306 0.002177
B 48.3 0.002854 0.003137
C 45.7 0.003377 0.00318
D 42.4 0.003411 0.00372 0.003891
E 37.7 0.02 0.02 0.02
F 33.6 0.03 0.03
G 31.3 0.03 0.03 0.03
H 30.0 0.03 0.03

Protein purified on 10/7/2022 and assayed on 10/28/2022.
Data uploaded by enielse and last updated on 2022-11-18 11:39:14.


Design Data

2.785

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.