D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant S302K

Expression Data

Yield (mg/mL): 4.207

Kinetic Assay Data

**slope of S302K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.84E-01	1.83E-01	1.80E-01
B	25.00	1.48E-01	1.45E-01	1.48E-01
C	8.33	1.08E-01	1.08E-01	1.09E-01
D	2.78	7.29E-02	6.18E-02	6.86E-02
E	0.93	3.18E-02	2.88E-02	3.05E-02
F	0.31	1.20E-02	1.14E-02	1.13E-02
G	0.10	4.65E-03	4.05E-03	4.35E-03
H	0.03	6.00E-04		6.00E-04

Yield (mg/mL): 4.207 Dilution Factor: 100×

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by AmandaH2308 and last updated on 2022-11-18 12:34:49.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3069	0.3233	0.3195
B	25.00	0.2660	0.2668	0.2742
C	8.33	0.1985	0.2022	0.2011
D	2.78	0.1112	0.1280	0.1002
E	0.93	0.04740	0.05040	0.05835
F	0.31	0.02115	0.01935	0.01905
G	0.10	0.007050	0.006900	0.007650
H	0.03	0.0006000	0.0006000	0

Yield (mg/mL): 6.509 Dilution Factor: 100×

Protein purified on 10/21/2022 and assayed on 10/28/2022.
Data uploaded by Ocapu001 and last updated on 2022-11-18 12:34:22.

Thermostability Assay Data

**slope of S302K A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	7.50E-04		9.00E-04
B	48.3
C	45.7	7.50E-04	9.00E-04	7.50E-04
D	42.4	2.40E-03	2.40E-03
E	37.7	8.66E-02	8.73E-02	8.91E-02
F	33.6	7.76E-02	7.94E-02	8.10E-02
G	31.3	1.25E-01	1.24E-01	1.12E-01
H	30.0	1.23E-01	1.28E-01

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by AmandaH2308 and last updated on 2024-08-01 14:57:47.

Reference Data for WT

**slope of S302K A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	7.50E-04	6.00E-04
B	48.0	6.00E-04	0	4.50E-04
C	46.0	6.00E-04	1.05E-03	7.50E-04
D	42.0	6.60E-03	6.45E-03	5.85E-03
E	38.0	3.74E-02	3.84E-02	3.56E-02
F	34.0	4.25E-02	4.16E-02	4.13E-02
G	31.0	4.98E-02	5.04E-02	4.80E-02
H	30.0	4.10E-02	4.35E-02	3.98E-02

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by Seaotter01 and last updated on 2022-11-18 08:12:14.

Design Data

Rosetta Score for S302K: 9.819

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.