Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 6.509

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.3069	0.3233	0.3195
	B	25.00	0.2660	0.2668	0.2742
	C	8.33	0.1985	0.2022	0.2011
	D	2.78	0.1112	0.1280	0.1002
	E	0.93	0.04740	0.05040	0.05835
	F	0.31	0.02115	0.01935	0.01905
	G	0.10	0.007050	0.006900	0.007650
	H	0.03	0.0006000	0.0006000	0

Yield (mg/mL): 6.509     Dilution Factor:  100×

Download these data

Protein purified on 10/21/2022 and assayed on 10/28/2022.
Data uploaded by Ocapu001 and last updated on 2022-11-18 12:34:22.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.0009000	0	0
	B	48.3
	C	45.7	0.0007500	0.0007500	0.0006000
	D	42.4	0.01065	0.01125	0.01095
	E	37.7	0.1248	0.1356	0.1358
	F	33.6	0.1686	0.1659	0.1731
	G	31.2	0.1714	0.1729	0.1785
	H	30.0	0.1814	0.1899	0.1806

Download these data

Protein purified on 10/21/2022 and assayed on 10/28/2022.
Data uploaded by Ocapu001 and last updated on 2022-11-18 12:42:57.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.