D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220Q

Yield (mg/mL): 0.291

**slope of N220Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.00E-02		1.00E-02
B	25.00	9.05E-03	9.31E-03	9.98E-03
C	8.33	6.31E-03	6.53E-03	6.53E-03
D	2.78	3.91E-03	3.44E-03	3.81E-03
E	0.93	2.18E-03	2.88E-03	1.82E-03
F	0.31	1.44E-03	1.15E-03	1.18E-03
G	0.10	2.13E-03	4.63E-03	1.02E-03
H	0.03	7.84E-04		8.32E-04

Yield (mg/mL): 0.291 Dilution Factor: 100×

Protein purified on 10/7/2022 and assayed on 10/21/2022.
Data uploaded by adworsky1 and last updated on 2022-11-18 11:57:06.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.09	0.1	0.09
B	25.00	0.07	0.06	0.06
C	8.33	0.05	0.05	0.05
D	2.78	0.03	0.03	0.03
E	0.93		0.01	0.01
F	0.31	0.006586	0.005952	0.005693
G	0.10	0.003252	0.003259	0.002836
H	0.03	0.000920455	0.000968182

Yield (mg/mL): 0.309 Dilution Factor: 100×

Protein purified on 10/7/2022 and assayed on 10/21/2022.
Data uploaded by enielse and last updated on 2022-11-22 16:31:57.

Data missing or deleted from the database.

**slope of N220Q A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0.002409	0.002743	0.003557
B	48.3	0.00228		0.00246
C	45.7	0.002974	0.002409	0.004294
D	42.4	0.003746	0.0039
E	37.7		0.03	0.03
F	33.6		0.04	0.04
G	31.3	0.03	0.03	0.03
H	30.0	0.04	0.04	0.05

Protein purified on 10/7/2022 and assayed on 10/28/2022.
Data uploaded by enielse and last updated on 2022-11-18 11:55:09.

Rosetta Score for N220Q: 2.572

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.