Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.309

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.07	0.09	0.07
	B	25.00	0.06	0.06	0.06
	C	8.33	0.04	0.04	0.04
	D	2.78	0.03	0.03	0.03
	E	0.93	0.01	0.01
	F	0.31	0.006048	0.006286	0.0063
	G	0.10	0.002959	0.002768	0.003075
	H	0.03	0.001711	0.001057	0.000456818

Yield (mg/mL): 0.309     Dilution Factor:  100×

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Protein purified on 10/7/2022 and assayed on 10/21/2022.
Data uploaded by enielse and last updated on 2022-11-22 16:29:10.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.001671	0.001303
	B	48.3	0.002391		0.002151
	C	45.7	0.001303		0.001294
	D	42.4	0.002546	0.003746	0.003154
	E	37.7		0.02	0.02
	F	33.6	0.03	0.03	0.03
	G	31.3		0.04	0.04
	H	30.0		0.04	0.04

Download these data

Protein purified on 10/7/2022 and assayed on 10/28/2022.
Data uploaded by enielse and last updated on 2022-11-18 11:47:10.

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.