Raw Data for BglB Variant T175H

Expression Data

SDS-PAGE
 0.0446

Kinetic Assay Data

slope of T175H A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 5.00E-02 5.00E-02 5.00E-02
B 25.00 3.00E-02 4.00E-02 3.00E-02
C 8.33 3.00E-02 3.00E-02
D 2.78 2.00E-02 2.00E-02 2.00E-02
E 0.93 7.51E-03 7.41E-03 8.10E-03
F 0.31 3.15E-03 3.07E-03 3.36E-03
G 0.10 2.52E-03 2.20E-03 2.34E-03
H 0.03 1.66E-03 1.37E-03
 0.0446      10×

Protein purified on 10/21/2022 and assayed on 10/21/2022.
Data uploaded by schiappetta1 and last updated on 2022-11-18 11:59:29.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.09 0.1 0.09
B 25.00 0.07 0.06 0.06
C 8.33 0.05 0.05 0.05
D 2.78 0.03 0.03 0.03
E 0.93 0.01 0.01
F 0.31 0.006586 0.005952 0.005693
G 0.10 0.003252 0.003259 0.002836
H 0.03 0.000920455 0.000968182
 0.309      100×

Protein purified on 10/7/2022 and assayed on 10/21/2022.
Data uploaded by enielse and last updated on 2022-11-22 16:31:57.


Thermostability Assay Data

slope of T175H A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00258 0.002863
B 48.3 0.001234 0.001491
C 45.7 0.00186 0.0015
D 42.4 0.003866 0.005366 0.002974
E 37.7 0.009746 0.007474 0.008983
F 33.6 0.01 0.01
G 31.3 0.01 0.01
H 30.0 0.02 0.02

Protein purified on 10/7/2022 and assayed on 10/28/2022.
Data uploaded by nkoniali and last updated on 2022-11-18 12:27:46.


Reference Data for WT

slope of T175H A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.002409 0.002743 0.003557
B 48.3 0.00228 0.00246
C 45.7 0.002974 0.002409 0.004294
D 42.4 0.003746 0.0039
E 37.7 0.03 0.03
F 33.6 0.04 0.04
G 31.3 0.03 0.03 0.03
H 30.0 0.04 0.04 0.05

Protein purified on 10/7/2022 and assayed on 10/28/2022.
Data uploaded by enielse and last updated on 2022-11-18 11:55:09.


Design Data

10.003

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.