D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220H

Expression Data

Yield (mg/mL): 7.457

Kinetic Assay Data

**slope of N220H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0324	0.03045	0.03345
B	25.00	0.0303	0.03015	0.0309
C	8.33	0.0225	0.0243	0.0225
D	2.78	0.0132	0.01245	0.0132
E	0.93	0.0057	0.00585	0.0048
F	0.31	0.00195	0.0024	0.0024
G	0.10	0.0012	0.0012	0.0012
H	0.03	0.00075	0.0006	0.00045

Yield (mg/mL): 7.457 Dilution Factor: 100×

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by jmend261 and last updated on 2022-11-18 08:05:43.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.04E-01	3.80E-01	3.70E-01
B	25.00	3.16E-01	3.03E-01	3.26E-01
C	8.33	2.55E-01	2.54E-01	2.48E-01
D	2.78	2.22E-01	2.19E-01	2.24E-01
E	0.93	1.29E-01	1.11E-01	1.13E-01
F	0.31	3.99E-02	3.93E-02	3.63E-02
G	0.10	1.65E-02	1.40E-02	2.01E-02
H	0.03	0	0	4.50E-04

Yield (mg/mL): 9.881 Dilution Factor: 100×

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by Seaotter01 and last updated on 2022-12-02 10:32:31.

Thermostability Assay Data

**slope of N220H A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	0	4.50E-04
B	48.0	7.50E-04	4.50E-04	0
C	46.0	6.00E-04		6.00E-04
D	42.0	1.20E-03	1.20E-03	1.20E-03
E	38.0	1.05E-02	1.08E-02	1.01E-02
F	34.0	1.49E-02	1.55E-02	1.37E-02
G	31.0	1.58E-02	1.68E-02	1.52E-02
H	30.0	1.74E-02	1.64E-02	1.55E-02

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by jmend261 and last updated on 2022-11-18 08:12:33.

Reference Data for WT

**slope of N220H A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	7.50E-04	6.00E-04
B	48.0	6.00E-04	0	4.50E-04
C	46.0	6.00E-04	1.05E-03	7.50E-04
D	42.0	6.60E-03	6.45E-03	5.85E-03
E	38.0	3.74E-02	3.84E-02	3.56E-02
F	34.0	4.25E-02	4.16E-02	4.13E-02
G	31.0	4.98E-02	5.04E-02	4.80E-02
H	30.0	4.10E-02	4.35E-02	3.98E-02

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by Seaotter01 and last updated on 2022-11-18 08:12:14.

Design Data

Rosetta Score for N220H: -0.048

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.