D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220G

Expression Data

Yield (mg/mL): 10.31

Kinetic Assay Data

**slope of N220G A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.02775	0.03105	0.03285
B	25.00	0.0171	0.01845	0.0201
C	8.33	0.00915	0.00945	0.00945
D	2.78	0.00435	0.00465	0.0042
E	0.93	0.00195	0.00195	0.00195
F	0.31	0.00105	0.0009	0.0009
G	0.10	0.00075	0.0006	0.0006
H	0.03	0.00105	0	0.0006

Yield (mg/mL): 10.31 Dilution Factor: 100×

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by celinamontero and last updated on 2022-11-18 08:11:13.

Thermostability Assay Data

**slope of N220G A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0	0
B	48.3	0.0004501	0.0006
C	45.7	0.0006		0.0007502
D	42.4	0.0006	0.00045
E	37.7	0.0012		0.0012
F	33.6	0.0036	0.00375	0.0039
G	31.3	0.0048	0.00525	0.00465
H	30.0	0.0045	0.0048	0.00465

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by celinamontero and last updated on 2024-08-08 18:34:39.

Reference Data for WT

**slope of N220G A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	7.50E-04	6.00E-04
B	48.0	6.00E-04	0	4.50E-04
C	46.0	6.00E-04	1.05E-03	7.50E-04
D	42.0	6.60E-03	6.45E-03	5.85E-03
E	38.0	3.74E-02	3.84E-02	3.56E-02
F	34.0	4.25E-02	4.16E-02	4.13E-02
G	31.0	4.98E-02	5.04E-02	4.80E-02
H	30.0	4.10E-02	4.35E-02	3.98E-02

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by Seaotter01 and last updated on 2022-11-18 08:12:14.

Design Data

Rosetta Score for N220G: 5.225

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.