Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 9.881

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	4.04E-01	3.80E-01	3.70E-01
	B	25.00	3.16E-01	3.03E-01	3.26E-01
	C	8.33	2.55E-01	2.54E-01	2.48E-01
	D	2.78	2.22E-01	2.19E-01	2.24E-01
	E	0.93	1.29E-01	1.11E-01	1.13E-01
	F	0.31	3.99E-02	3.93E-02	3.63E-02
	G	0.10	1.65E-02	1.40E-02	2.01E-02
	H	0.03	0	0	4.50E-04

Yield (mg/mL): 9.881     Dilution Factor:  100×

Download these data

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by Seaotter01 and last updated on 2022-12-02 10:32:31.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	6.00E-04	7.50E-04	6.00E-04
	B	48.0	6.00E-04	0	4.50E-04
	C	46.0	6.00E-04	1.05E-03	7.50E-04
	D	42.0	6.60E-03	6.45E-03	5.85E-03
	E	38.0	3.74E-02	3.84E-02	3.56E-02
	F	34.0	4.25E-02	4.16E-02	4.13E-02
	G	31.0	4.98E-02	5.04E-02	4.80E-02
	H	30.0	4.10E-02	4.35E-02	3.98E-02

Download these data

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by Seaotter01 and last updated on 2022-11-18 08:12:14.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.