Raw Data for BglB Variant N220R

Expression Data

SDS-PAGE
 7.231

Kinetic Assay Data

slope of N220R A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 5.70E-03 5.40E-03 5.55E-03
B 25.00 3.75E-03 4.05E-03 4.05E-03
C 8.33 2.40E-03 2.25E-03 2.40E-03
D 2.78 1.50E-03 1.50E-03 1.50E-03
E 0.93 1.05E-03 9.00E-04 9.00E-04
F 0.31 9.00E-04 6.00E-04 7.50E-04
G 0.10 6.00E-04 6.00E-04
H 0.03 0 0 0
 7.231      100×

Protein purified on 10/21/2022 and assayed on 10/28/2022.
Data uploaded by aam71201 and last updated on 2022-11-18 07:47:44.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.13E-01 1.10E-01 1.11E-01
B 25.00 9.09E-02 8.88E-02 9.15E-02
C 8.33 6.57E-02 6.83E-02 6.77E-02
D 2.78 3.86E-02 3.65E-02 3.95E-02
E 0.93 2.24E-02 2.40E-02 2.28E-02
F 0.31 1.02E-02 9.75E-03 1.02E-02
G 0.10 4.80E-03 4.20E-03 5.25E-03
H 0.03 6.00E-04 7.50E-04
 2.294      100×

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by mayoral and last updated on 2022-11-18 08:08:58.


Thermostability Assay Data

slope of N220R A420 nm (min−1)
T (°C) 1 2 3
A 50.0 6.00E-04 4.50E-04
B 48.3
C 45.7 4.50E-04 6.00E-04
D 42.4 7.50E-04 6.00E-04 6.00E-04
E 37.7 2.10E-03 1.80E-03 1.65E-03
F 33.6 2.10E-03 1.95E-03 1.95E-03
G 31.3 1.95E-03 1.95E-03
H 30.0 1.80E-03

Protein purified on 10/21/2022 and assayed on 10/28/2022.
Data uploaded by aam71201 and last updated on 2022-11-18 08:36:43.


Reference Data for WT

slope of N220R A420 nm (min−1)
T (°C) 1 2 3
A 50.0 6.00E-04 7.50E-04 6.00E-04
B 48.3 6.00E-04 4.50E-04
C 45.7 6.00E-04 1.05E-03 7.50E-04
D 42.4 6.60E-03 6.45E-03 5.85E-03
E 37.7 3.74E-02 3.84E-02 3.56E-02
F 33.6 4.25E-02 4.16E-02 4.13E-02
G 31.3 4.98E-02 5.04E-02 4.80E-02
H 30.0 4.10E-02 4.35E-02 3.98E-02

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by mayoral and last updated on 2022-11-18 08:16:50.


Design Data

-14.181

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.