D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220M

Expression Data

Yield (mg/mL): 7.505

Kinetic Assay Data

**slope of N220M A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.14E-01	2.24E-01	2.24E-01
B	25.00	2.09E-01	2.29E-01	2.12E-01
C	8.33	1.82E-01	1.95E-01	1.90E-01
D	2.78	1.36E-01	1.45E-01	1.37E-01
E	0.93	9.05E-02	9.59E-02	8.66E-02
F	0.31	4.55E-02	4.53E-02	4.82E-02
G	0.10	2.25E-02	2.18E-02	2.27E-02
H	0.03	4.50E-04	6.00E-04	0

Yield (mg/mL): 7.505 Dilution Factor: 100×

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by jessicamorier and last updated on 2022-11-18 07:49:13.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.13E-01	1.10E-01	1.11E-01
B	25.00	9.09E-02	8.88E-02	9.15E-02
C	8.33	6.57E-02	6.83E-02	6.77E-02
D	2.78	3.86E-02	3.65E-02	3.95E-02
E	0.93	2.24E-02	2.40E-02	2.28E-02
F	0.31	1.02E-02	9.75E-03	1.02E-02
G	0.10	4.80E-03	4.20E-03	5.25E-03
H	0.03	6.00E-04		7.50E-04

Yield (mg/mL): 2.294 Dilution Factor: 100×

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by mayoral and last updated on 2022-11-18 08:08:58.

Thermostability Assay Data

**slope of N220M A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	6.00E-04	7.50E-04
B	48.3		7.50E-04	6.00E-04
C	45.7	7.50E-04	6.00E-04	7.50E-04
D	42.4	2.40E-03	2.40E-03
E	37.7	6.05E-02	5.96E-02	5.57E-02
F	33.6	1.39E-01	1.45E-01	1.31E-01
G	31.3	1.25E-01	1.49E-01	1.37E-01
H	30.0		1.39E-01

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by jessicamorier and last updated on 2024-08-08 18:40:01.

Reference Data for WT

**slope of N220M A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	7.50E-04	6.00E-04
B	48.3	6.00E-04		4.50E-04
C	45.7	6.00E-04	1.05E-03	7.50E-04
D	42.4	6.60E-03	6.45E-03	5.85E-03
E	37.7	3.74E-02	3.84E-02	3.56E-02
F	33.6	4.25E-02	4.16E-02	4.13E-02
G	31.3	4.98E-02	5.04E-02	4.80E-02
H	30.0	4.10E-02	4.35E-02	3.98E-02

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by mayoral and last updated on 2022-11-18 08:16:50.

Design Data

Rosetta Score for N220M: 1.044

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.