D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N404E

Expression Data

Yield (mg/mL): 1.962

Kinetic Assay Data

**slope of N404E A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.40E-03	1.65E-03	1.60E-03
B	25.00	1.45E-03	1.40E-03	1.35E-03
C	8.33	9.00E-04	9.00E-04
D	2.78	7.50E-04		7.50E-04
E	0.93	4.00E-04	4.50E-04
F	0.31	4.00E-04	4.00E-04	3.50E-04
G	0.10	2.50E-04	0	5.50E-04
H	0.03	5.00E-04	5.00E-04	5.50E-04

Yield (mg/mL): 1.962 Dilution Factor: 10×

Protein purified on 11/9/2022 and assayed on 11/10/2022.
Data uploaded by BryantLuu and last updated on 2022-11-19 15:12:54.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.29E-01	1.22E-01	1.60E-01
B	25.00	1.31E-01	9.52E-02	1.83E-01
C	8.33	1.07E-01	7.84E-02	9.78E-02
D	2.78	7.70E-02	5.26E-02	4.06E-02
E	0.93	2.35E-02	3.12E-02	2.49E-02
F	0.31	9.70E-03	9.75E-03	1.06E-02
G	0.10	3.95E-03	4.95E-03	3.40E-03
H	0.03	3.50E-04	0	0

Yield (mg/mL): 1.306 Dilution Factor: 100×

Protein purified on 11/9/2022 and assayed on 11/10/2022.
Data uploaded by BryantLuu and last updated on 2022-11-19 15:18:00.

Thermostability Assay Data

**slope of N404E A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	5.00E-04	3.00E-04	4.00E-04
B	48.3	0	0	3.50E-04
C	45.7	1.90E-03	2.50E-04	8.50E-04
D	42.4	6.50E-04	2.50E-04	4.50E-04
E	37.7	7.00E-04		7.00E-04
F	33.6	8.00E-04		7.50E-04
G	31.3	8.50E-04	7.00E-04	8.00E-04
H	30.0	7.50E-04	7.00E-04	8.00E-04

Protein purified on 11/9/2022 and assayed on 11/10/2022.
Data uploaded by BryantLuu and last updated on 2022-11-20 14:42:29.

Reference Data for WT

**slope of N404E A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	4.00E-04	3.00E-04
B	48.3	4.00E-04	0	4.00E-04
C	45.7	1.15E-03	6.50E-04	8.00E-04
D	42.4	1.46E-02	1.36E-02	1.43E-02
E	37.7	5.10E-02	4.49E-02	4.02E-02
F	33.6	5.34E-02	5.29E-02	5.42E-02
G	31.3	6.68E-02	5.32E-02	6.24E-02
H	30.0	6.62E-02	5.58E-02	5.45E-02

Protein purified on 11/9/2022 and assayed on 11/10/2022.
Data uploaded by BryantLuu and last updated on 2022-11-20 14:40:06.

Design Data

Rosetta Score for N404E: 4.714

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Peishan: <peihuang@ucdavis.edu>.