D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Q124P

Expression Data

Yield (mg/mL): 0.75

Kinetic Assay Data

**slope of Q124P A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0625	0.06075	0.06395
B	25.00	0.0597	0.0596	0.0676
C	8.33	0.0442	0.0423	0.0451
D	2.78	0.0245	0.02525	0.0251
E	0.93	0.008601	0.00945	0.00955
F	0.31	0.0035	0.00325	0.0035
G	0.10	0.00145	0.0015	0.0013
H	0.03		0.0003	0.00025

Yield (mg/mL): 0.75 Dilution Factor: 100×

Protein purified on 11/2/2022 and assayed on 11/9/2022.
Data uploaded by nbraga and last updated on 2022-11-17 21:18:08.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.05005	0.05545	0.0509
B	25.00	0.0451	0.05685	0.0494
C	8.33	0.04285	0.0428	0.041
D	2.78	0.01635	0.01995	0.0179
E	0.93	0.0095	0.008151	0.00845
F	0.31	0.00395	0.0033	0.00235
G	0.10		0.0014	0.00125
H	0.03	0	0.0002	0.00035

Yield (mg/mL): 0.56 Dilution Factor: 100×

Protein purified on 11/2/2022 and assayed on 11/9/2022.
Data uploaded by jannahal and last updated on 2022-11-18 19:11:00.

Thermostability Assay Data

**slope of Q124P A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0	0	0
B	48.3	0	0.0004	0.0005
C	45.7	0.0009	0.001	0.0007
D	42.4	0.0037	0.004	0.0044
E	37.7	0.0178	0.0175
F	33.6	0.0187	0.0171	0.0178
G	31.3	0.0253	0.0217	0.0237
H	30.0	0.0241	0.0221	0.0233

Protein purified on 11/2/2022 and assayed on 11/9/2022.
Data uploaded by nbraga and last updated on 2022-11-17 21:28:37.

Reference Data for WT

**slope of Q124P A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0	0	0
B	48.3	0	0	0
C	45.7	0	0.0007	0.0006
D	42.4	0.0019	0.0021
E	37.7	0.0124	0.0135	0.0109
F	33.6	0.012	0.0149	0.0157
G	31.3	0.0101	0.013	0.0164
H	30.0		0.0084	0.0085

Protein purified on 11/2/2022 and assayed on 11/9/2022.
Data uploaded by jannahal and last updated on 2022-11-18 19:16:13.

Design Data

Rosetta Score for Q124P: 335.995

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.