Raw Data for BglB Variant Q124T

Expression Data

SDS-PAGE
 1.3

Kinetic Assay Data

slope of Q124T A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 6.06E-02 6.25E-02 6.51E-02
B 25.00 6.52E-02 6.18E-02 5.69E-02
C 8.33 3.93E-02 4.13E-02 3.89E-02
D 2.78 2.33E-02 2.28E-02 2.18E-02
E 0.93 1.06E-02 1.00E-02 1.04E-02
F 0.31 4.35E-03 3.95E-03 3.90E-03
G 0.10 1.55E-03 1.60E-03 1.15E-03
H 0.03 6.50E-04 8.50E-04 8.50E-04
 1.3      100×

Protein purified on and assayed on .
Data uploaded by scisneros and last updated on 2022-11-16 21:48:59.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.1927 0.1925 0.2163
B 25.00 0.1763 0.1585 0.1588
C 8.33 0.1211 0.1249 0.1223
D 2.78 0.0744 0.06635
E 0.93 0.02975 0.02955 0.0315
F 0.31 0.01185 0.01125
G 0.10 0.00555 0.0049 0.0055
H 0.03 0.0018 0.00195
 1.79      100×

Protein purified on 11/2/22 and assayed on 11/9/22.
Data uploaded by jeremiah and last updated on 2022-11-16 11:40:17.


Thermostability Assay Data

slope of Q124T A420 nm (min−1)
T (°C) 1 2 3
A 50.0 5.00E-04 5.00E-04
B 47.1 4.00E-04 8.00E-04 7.00E-04
C 44.3 6.00E-04 5.00E-04 5.00E-04
D 41.4 1.50E-03 1.40E-03
E 38.6 6.50E-03 7.20E-03 7.60E-03
F 35.7 9.50E-03 9.10E-03 9.00E-03
G 32.9 1.02E-02 8.90E-03 9.20E-03
H 50.0 7.50E-03 8.50E-03

Protein purified on and assayed on .
Data uploaded by scisneros and last updated on 2022-11-16 21:55:55.


Reference Data for WT

slope of Q124T A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0007 0.0007 0.0007
B 48.3 0.0005 0.0004
C 45.7 0.0011 0.0012
D 42.4 0.0286 0.0312 0.0321
E 37.7 0.1001 0.1097 0.1059
F 33.6 0.112 0.1139 0.112
G 31.3 0.1265 0.0987 0.1287
H 30.0 0.1035 0.1305

Protein purified on 11/2/22 and assayed on 11/9/22.
Data uploaded by jeremiah and last updated on 2022-11-16 12:05:29.


Design Data

7.519

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.