D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N404L

Expression Data

Yield (mg/mL): 1.993

Kinetic Assay Data

**slope of N404L A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.56E-03	6.48E-03	6.84E-03
B	25.00	3.90E-03	3.87E-03	3.89E-03
C	8.33	2.05E-03	2.15E-03	2.31E-03
D	2.78	8.30E-04	8.34E-04	8.17E-04
E	0.93	3.52E-04	3.44E-04	2.55E-04
F	0.31	1.64E-04	1.50E-04	2.07E-04
G	0.10	1.60E-04	8.91E-05	1.07E-04
H	0.03	5.33E-05	0	2.30E-05

Yield (mg/mL): 1.993 Dilution Factor: 100×

Protein purified on 04-10-2022 and assayed on 05-10-2022.
Data uploaded by ReetS and last updated on 2022-10-27 13:21:17.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.78E-01	1.84E-01	2.03E-01
B	25.00	1.53E-01	1.65E-01	1.69E-01
C	8.33	1.32E-01	1.24E-01	1.24E-01
D	2.78	7.45E-02	7.41E-02	8.29E-02
E	0.93	3.50E-02	3.63E-02	4.34E-02
F	0.31	1.34E-02	1.32E-02	1.58E-02
G	0.10	5.38E-03	5.40E-03	6.50E-03
H	0.03	1.52E-05	0	3.70E-05

Yield (mg/mL): 2.905 Dilution Factor: 100×

Protein purified on 04-10-2022 and assayed on 05-10-2022.
Data uploaded by ReetS and last updated on 2022-10-27 15:22:31.

Design Data

Rosetta Score for N404L: -1.473

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.