D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T13S

Expression Data

Yield (mg/mL): 1.51

Kinetic Assay Data

**slope of T13S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.75E-01	1.83E-01	1.75E-01
B	25.00	1.51E-01	1.55E-01	1.44E-01
C	8.33	1.52E-01	1.56E-01	1.52E-01
D	2.78	8.34E-02	9.34E-02	9.08E-02
E	0.93	3.19E-02	3.48E-02	3.46E-02
F	0.31	1.11E-02	1.21E-02	1.12E-02
G	0.10	3.74E-03	4.24E-03	4.02E-03
H	0.03	2.30E-05	0	0

Yield (mg/mL): 1.51 Dilution Factor: 100×

Protein purified on 10/3/2022 and assayed on 10/4/2022.
Data uploaded by tgannalo and last updated on 2022-10-21 15:06:53.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.24E-01	1.32E-01	1.29E-01
B	25.00	1.30E-01	1.35E-01	1.32E-01
C	8.33	9.85E-02	1.08E-01	9.15E-02
D	2.78	6.21E-02	6.81E-02	6.40E-02
E	0.93	2.98E-02	3.26E-02	3.09E-02
F	0.31	1.21E-02	1.21E-02	1.18E-02
G	0.10	3.36E-03	3.82E-03	3.69E-03
H	0.03	0	1.24E-04	3.15E-05

Yield (mg/mL): 1.17 Dilution Factor: 100×

Protein purified on 10/3/2022 and assayed on 10/4/2022.
Data uploaded by tgannalo and last updated on 2022-10-21 15:10:56.

Design Data

Rosetta Score for T13S: 2.828

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Peishan: <peihuang@ucdavis.edu>.